Senior Cytogenetics Technologist - Genomic Stability

Responsibilities

• Transfer in, implement, and document an established G-banding and karyotyping workflow in-house for HSC program cell products.
• Execute the full cytogenetics workflow (initially in research/PD and then in our GMP suite): metaphase arrest, cell harvest, hypotonic treatment, slide preparation, fixation, Giemsa staining, metaphase imaging, and software-assisted karyotype analysis.
• Compare initial in-house results against historical data and document appropriate operating and quality-control criteria.
• Analyze metaphase spreads to identify numerical and structural chromosomal abnormalities, and document findings clearly.

Support genomic-stability assessment of human primary cells

• Culture, passage, expand, bank, and prepare iPSC samples in preparation for karyotyping
• Track clone-level genomic stability, passage number, culture conditions, and QC status across lines.
• Coordinate with CROs shipments of samples for genomic instability analysis
• Maintain organized records, sample tracking, and imaging files, and develop SOPs for the workflows you build.
• Are comfortable with high-magnification microscopy.
• Are highly organized and able to run multiple clone/sample workflows in parallel.

Preferred

• Hold current CG(ASCP) certification in cytogenetics.
• Hold a Bachelor's or Master's degree in cell biology, molecular biology, cytogenetics, genetics, stem cell biology, biomedical sciences, or a related field.

About you

You're a careful, hands-on technologist who takes pride in high-quality chromosome preparations and confident karyotype calls. You are comfortable bringing an established cytogenetics workflow into a new laboratory environment—setting up the process, confirming that it performs consistently, documenting it clearly, and troubleshooting issues as they arise. You're at home at the microscope and equally happy in a tissue-culture hood keeping iPSC lines healthy. You're collaborative, easy-going, proactive in how you communicate, and flexible about scheduling when the culture rotation calls for it.

What you'll do in this role

You might thrive in this role if you

Have several years of hands-on G-banding and karyotype analysis experience, with demonstrated ability to make independent chromosome assessments and troubleshoot chromosome preparation and image quality.

Have experience with imaging software such as MetaSystems / Ikaros or Cytovision.

Have hands-on mammalian cell culture experience with strong sterile technique.

Are available for weekend work as part of a team rotation.

Bonus if you

• Have experience standing up or validating a new assay in-house.
• Have hands-on experience maintaining iPSC cultures.
• At Retro, we don't use titles. New hires join as a "Member of X Team." The expectations for this role are generally aligned to the level of Cytogenetics Technologist.
• Total compensation also includes generous equity and benefits including:
• Medical, dental, and vision insurance for you and your family

401(k) plan with 4% matching

Unlimited time off and 10 company holidays per year

Annual learning & development stipend

Free lunch and snacks

We take pride in cultivating an environment that fosters collaboration, open communication, and authenticity. We are an equal opportunity employer; we do not discriminate on the basis of race, religion, color, national origin, gender, sexual orientation, age, marital status, veteran status, or disability status.